I D
× COMMENTARYEDITOR'S PAGECOVER STORYIN THE NEWSNEWSFRONTSCHEMENTATOR + Show More BUSINESS NEWSTECHNICAL & PRACTICALFEATURE REPORTFACTS AT YOUR FINGERTIPSTECHNOLOGY PROFILEENVIRONMENTAL MANAGEREQUIPMENT & SERVICESFOCUSNEW PRODUCTS + Show More

Comment uncategorized

Immobilizing enzymes for biosensors

By Paul Grad |

A new technique for making less-expensive, more-efficient biological enzyme hybrids could have widespread applications, including in water recycling, drug manufacturing and molecular biology. The technique, developed by a team from Australia and the U.S., involves using a vortex fluidic device (VFD) to immobilize an enzyme hybrid, making it possible to reuse the enzyme under continuous flow.

The team, which includes professor Colin Raston from Flinders University (Adelaide, Australia; www.flinders.edu.au) and professor Gregory Weiss from the University of California at Irvine (www.uci.edu), reported details of a low-cost VFD that has a rapidly rotating tube open at one end. At high rotational speed, intense shear is generated at the open end in the resulting thin films. The device can also operate under a continuous-flow mode with jet feeds delivering liquid into the rotating tube, where additional shear is generated.

Harnessing the high shear forces and micro mixing in a VFD resulted in a dramatically accelerated fabrication of hybrid protein-Cu3(PO4)2 “nanoflowers.” The team was able to generate and immobilize the nanoflowers into silica hydrogel. This greatly simplified the fabrication process, and allowed reusing the enzyme. It also increased the enzyme’s catalytic rates by 16 times. This technique overcomes some of the drawbacks of using enzymes in biosensors, such as cost and limited life of the enzymes — most enzymes become inactive during the assay process and cannot be reused.

The study was published in the November 5, 2020 issue of the ACS journal, Applied Materials & Interfaces.

Related Content
A catalyst that mimics enzymes
A research team from the University of New South Wales (Sydney, Australia; www.unsw.edu.au) and Ruhr-Universität Bochum (Bochum, Germany; www.ruhr-universität-bochum.de) has…

Chemical Engineering publishes FREE eletters that bring our original content to our readers in an easily accessible email format about once a week.
Subscribe Now
Video - CoriolisMaster
Video - Do you really need a thermowell?
The influence of IIoT in the dewatering process step of pigment production
The Big 6 level measurement technologies, where to use them and why
Top five technologies for drying in chemical applications

View More